Optimization of single molecules axial location precision in 3D stochastic optical reconstruction microscopy

	Optimization of single molecules axial location precision in 3D stochastic optical reconstruction microscopy
	Zhang, S.-C.; S.-M. Li; G. Yang; H. Li and D.-X. Xiong
	2015
发表期刊	Guangzi Xuebao/Acta Photonica Sinica
卷号	44 期号:10
摘要	The relationship between the ellipticity of point spread function and the focal length of cylindrical lens was investigated. Fluorescent inverted microscopey imaging system is based on Olympus IX-83. With three different focal length, the point spread function with custom built stochastic optical reconstruction microscopy instruments was measured. A method to evaluate the cylindrical lens was developed based on linear region and localization error. The results show that linear region of 1 m and axial localization error of 16 nm can be achieved with correct focal length. As the demonstration, three dimension super-resolution image of Actin filaments was reconstructed. , 2015, Chinese Optical Society. All right reserved.
文章类型	期刊论文
收录类别	EI
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/56245
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Zhang, S.-C.,S.-M. Li,G. Yang,et al. Optimization of single molecules axial location precision in 3D stochastic optical reconstruction microscopy[J]. Guangzi Xuebao/Acta Photonica Sinica,2015,44(10).
APA	Zhang, S.-C.,S.-M. Li,G. Yang,&H. Li and D.-X. Xiong.(2015).Optimization of single molecules axial location precision in 3D stochastic optical reconstruction microscopy.Guangzi Xuebao/Acta Photonica Sinica,44(10).
MLA	Zhang, S.-C.,et al."Optimization of single molecules axial location precision in 3D stochastic optical reconstruction microscopy".Guangzi Xuebao/Acta Photonica Sinica 44.10(2015).

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