CD-Like微流控生化分析系统光探测技术研究

	CD-Like微流控生化分析系统光探测技术研究
其他题名	Studies on Optical Detection Technology of CD-Like Microfluidic Biochemical Analysis System
	王宇
学位类型	硕士
导师	吴一辉
	2015-10
学位授予单位	中国科学院大学
学位专业	光学工程
关键词	光探测微流控芯片生化分析
摘要	CD-like微流控芯片是一种简单高效的生化分析平台，它通过调整自身的转速实现对芯片内部微流体的操作和控制，具有试剂用量少、集成度高、分析速度快、体积小等优点。本论文为实现CD-Like微流控芯片多指标、多波长的探测目的，围绕芯片的光探测系统设计，展开了以下研究工作： 1. 采用闪烁氙灯作为光源，微型光谱仪作为检测器，设计并搭建了光探测系统。验证了多路光电检测电路，给出了芯片精确定位与多参数检测方法； 2. 对影响光探测系统性能的主要因素进行了分析研究，给出了误差源，建立了光源输出稳定性、杂散光与吸光度误差之间的关系；给出了以双波长检测为主体的光源输出抖动补偿方法并对其进行了实验验证，相比单波长检测方法，检测结果的重复性提高了18倍； 3. 以葡萄糖、总蛋白、白蛋白和二氧化碳结合力等生化指标的检测为目标，对光探测系统性能进行了研究。测试结果显示，定标曲线的线性度达到99%以上，吸光度线性范围、重复性和杂散光均满足国家标准。本论文的工作将有利于推进CD-Like微流控芯片在医学临床领域的应用，对实现生化分析仪器的集成化与小型化具有重要意义。
其他摘要	CD-like microfluidic chip is a simple and efficient biochemical analysis platform. It manipulates and controls the internal microfluidics by adjusting its own speed, which has the advantage of reagent consumption, high integration, high speed analysis, small size and so on. In this paper, to achieve the purpose of multi-index and multi-wavelength detection, studies on the design of light detection system is carried out. The main contents are as follows: 1. The optical detection system was designed and built by using flashing xenon lamp as light source and miniature spectrometer as detector. Multi-channel photoelectric detection system was verified, and method of positioning accurately and multi-parameter detection were given. 2. The main factors of affecting the performance of light detection system were analyzed. The relationship between output stability of light source and absorbance error and the relationship between stray light and absorbance error were established. The impact of differences on light output intensity was avoided by using dual wavelength absorbance detection method, and the test result showed that detection repeatability was 18 times higher than before. 3. The performance of optical detection system was investigated by testing four biochemical indices of glucose, total protein, albumin, carbon dioxide combining power. The test result showed the linearity of the calibration curve was more than 99%,and the light detection linear range, repeatability and stray light of the system met the national standards. The work in this paper is expected to promote the application of CD-Like microfluidic chip in clinical areas, and has great significance for integration and miniaturization of biochemical analysis instrument.
语种	中文
文献类型	学位论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/49327
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	王宇. CD-Like微流控生化分析系统光探测技术研究[D]. 中国科学院大学,2015.

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