用于兽药掺假检测的拉曼光谱仪光学系统设计

	用于兽药掺假检测的拉曼光谱仪光学系统设计
其他题名	Optical design of Raman spectrometer for veterinary drugs detection
	何振磊
学位类型	硕士
导师	卢启鹏
	2015-10
学位授予单位	中国科学院大学
学位专业	光学工程
关键词	拉曼光谱兽药检测光学设计透射式系统
摘要	兽药可以有效防治动物的疾病，对畜牧业的健康发展发挥着重要的作用。然而，一些企业在药品中非法掺杂其他物质冒充药物的有效成分，从而延误治疗，给养殖户造成了巨大的损失。这些掺杂成分与药品性状相似，肉眼难以分辨，需要专业的仪器进行检测。分析检测技术需要满足准确、快速、简便、经济等要求，其中任何一项的缺失，都会限制该技术的推广使用。目前，假药检测方法主要有化学法、色谱法和光谱法。其中，拉曼光谱法是以拉曼散射为基础的光谱分析技术，它具有速度快、非接触、无损伤、所需样品量少且无需预处理等优点，非常适合药品的现场快速检测。随着便携式拉曼光谱仪的出现和化学计量学方法的发展，拉曼光谱分析在药品检测方面的优势更加凸显。本文通过实验进行了兽药掺假的拉曼光谱检测，并设计了用于兽药掺假检测的拉曼光谱仪光学系统，主要内容包括：（1）本文通过拉曼光谱实验与化学计量学方法相结合，研究了兽药掺假的拉曼光谱分析方法，为拉曼光谱仪的设计提供实验指导。首先介绍了偏最小二乘法的定性判别和定量分析原理，然后以兽药中的常用药青霉素作为研究对象，基于偏最小二乘法，对掺葡萄糖和淀粉的青霉素样品进行了定性判别，并对掺假药品中的青霉素含量进行了定量分析。其中包括校正集的选取，奇异样本剔除以及各种预处理方法及其组合对模型的影响等。（2）本文根据兽药检测的需求，设计了便携式拉曼光谱仪的外光路系统。外光路系统包括激光激发和散射光收集两部分。激发光路与激光器输出光纤的数值孔径相匹配，放大率为1:1，系统的点列图大小与爱里斑相当，聚焦能力强。散射光收集光路的物方数值孔径达到0.44，能够使更大角度的拉曼散射光进入系统。所设计的散射光收集光路，零视场和50μm视场的点列图的均方根半径分别为 3.949μm和5.074μm，半径10μm的圆内能量超过95%。（3）本文设计了透射式体全息光栅分光的拉曼光谱仪光学系统。根据Kogelnik耦合波理论，分析了体全息光栅的衍射特性；设计了透射式拉曼光谱仪光学系统，该系统的长度小于10cm，物方数值孔径达到0.22，在790-1014nm的光谱范围内，分辨率为0.6nm。
其他摘要	Veterinary drugs can effectively control animal disease. They play an important role in promoting the healthy development of animal husbandry. However, some enterprises illegally adulterate veterinary drugs with other material, which has delayed treatment and caused huge losses to farmers. The bogus drugs are difficult to distinguish, so professional instruments are needed. Detection and analysis technique should be accurate, rapid, simple and cheap, or its promotion will be limited. Chemical method, chromatography and spectrometry are the main methods for bogus drugs detection at present. Raman spectroscopy is a kind of spectrometry based on Raman scattering. It is rapid, non-contact, nondestructive, and it requires less samples which needn’t to be pre-treated, so its suitable for bogus drugs detection. With the emergence of portable Raman spectrometer and the development of chemometrics, the advantages of Raman spectroscopy are highlighted. In this paper, Raman spectrum experiment is conducted to detecting adulterated veterinary drugs, and an optical system for Raman system is designed. The main contents of this paper are as follows: (1) Through a combination of Raman spectrum experiment and chemometrics, this paper studied the veterinary drugs detection based on Raman spectroscopy, which provided guidance for the design of Raman spectrometer. The basic theory of qualitative discrimination and quantitative analysis using partial least squares (PLS) is introduced. Qualitative and quantitative analysis is done of Penicillin adulterated with glucose and starch based on partial least squares. The research contains the establishment of calibration set, the elimination of singular sample, the impact analysis of different pretreatment and its combination, and so on. (2) Based on the requirement of veterinary drugs detection, this paper designed a Raman probe for portable Raman spectrometer. The Raman probe includes two parts of excitation and collection. The excitation system has a numerical aperture of 0.22, which matches with the fiber. Its magnification is 1:1. The radius of the spot diagram is almost the same as the Airy disk. The numerical aperture of the collection system reaches 0.44, which allows large-angle light to enter the system. The spot diagram of zero field of view and 50μm field of view are 3.949μm and 5.074μm respectively. The 10μm encircled energy exceeds 95%. (3) In this paper, an optical system for Raman spectrometer is designed using transmission volume holographic grating. The diffraction efficiency of the grating is analyzed based on Kogelnik’s coupled wave theory. A transmission optical system for Raman spectrometer is designed. The length of the system is less than 10cm. The numerical aperture reaches 0.22 and the resolution is 0.6nm in the range of 790-1014nm.
语种	中文
文献类型	学位论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/49267
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	何振磊. 用于兽药掺假检测的拉曼光谱仪光学系统设计[D]. 中国科学院大学,2015.

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