Changchun Institute of Optics,Fine Mechanics and Physics,CAS
Effect of particle size on conformation and enzymatic activity of EcoRI adsorbed on CdS nanoparticles | |
Song Y. H.; Zhong D. D.; Luo D.; Huang M.; Huang Z. Z.; Tan H. L.; Sun L. L.; Wang L. | |
2014 | |
发表期刊 | Colloids and Surfaces B-Biointerfaces |
ISSN | ISBN/0927-7765 |
期号 | 114页码:269-276 |
摘要 | Better understanding of the interaction between nanoparticles (NPs) and protein is the basis for biological and biomedical applications of NPs. Water-soluble fluorescent CdS NPs have been widely used in the biological and biomedical fields and the study on effect of CdS NPs size on conformation and enzymatic activity of protein might be very important in its application. In this work, the interaction of CdS NPs with different size with type II restriction endonuclease (EcoRI) were investigated by atomic force microscopy, transmission electron microscopy, UV-vis spectroscopy, fluorescence quenching method, CD spectra, laser scanning confocal microscopy and gel electrophoresis. It was found that the equilibrium constant (k(D)) as well as the cooperativity degree of CdS NPs-EcoRI binding (Hill constant, n) strongly depended on the CdS NPs size. The different curvature of CdS NPs surface could result in different changes of EcoRI conformation. The gel electrophoresis indicated that the decrease in alpha-helix content more or less affected the activity of EcoRI. (C) 2013 Elsevier B.V. All rights reserved. |
收录类别 | SCI ; EI |
语种 | 英语 |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ciomp.ac.cn/handle/181722/43932 |
专题 | 中科院长春光机所知识产出 |
推荐引用方式 GB/T 7714 | Song Y. H.,Zhong D. D.,Luo D.,et al. Effect of particle size on conformation and enzymatic activity of EcoRI adsorbed on CdS nanoparticles[J]. Colloids and Surfaces B-Biointerfaces,2014(114):269-276. |
APA | Song Y. H..,Zhong D. D..,Luo D..,Huang M..,Huang Z. Z..,...&Wang L..(2014).Effect of particle size on conformation and enzymatic activity of EcoRI adsorbed on CdS nanoparticles.Colloids and Surfaces B-Biointerfaces(114),269-276. |
MLA | Song Y. H.,et al."Effect of particle size on conformation and enzymatic activity of EcoRI adsorbed on CdS nanoparticles".Colloids and Surfaces B-Biointerfaces .114(2014):269-276. |
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