CIOMP OpenIR  > 中科院长春光机所知识产出
Effect of particle size on conformation and enzymatic activity of EcoRI adsorbed on CdS nanoparticles
Song Y. H.; Zhong D. D.; Luo D.; Huang M.; Huang Z. Z.; Tan H. L.; Sun L. L.; Wang L.
2014
发表期刊Colloids and Surfaces B-Biointerfaces
ISSNISBN/0927-7765
期号114页码:269-276
摘要Better understanding of the interaction between nanoparticles (NPs) and protein is the basis for biological and biomedical applications of NPs. Water-soluble fluorescent CdS NPs have been widely used in the biological and biomedical fields and the study on effect of CdS NPs size on conformation and enzymatic activity of protein might be very important in its application. In this work, the interaction of CdS NPs with different size with type II restriction endonuclease (EcoRI) were investigated by atomic force microscopy, transmission electron microscopy, UV-vis spectroscopy, fluorescence quenching method, CD spectra, laser scanning confocal microscopy and gel electrophoresis. It was found that the equilibrium constant (k(D)) as well as the cooperativity degree of CdS NPs-EcoRI binding (Hill constant, n) strongly depended on the CdS NPs size. The different curvature of CdS NPs surface could result in different changes of EcoRI conformation. The gel electrophoresis indicated that the decrease in alpha-helix content more or less affected the activity of EcoRI. (C) 2013 Elsevier B.V. All rights reserved.
收录类别SCI ; EI
语种英语
文献类型期刊论文
条目标识符http://ir.ciomp.ac.cn/handle/181722/43932
专题中科院长春光机所知识产出
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Song Y. H.,Zhong D. D.,Luo D.,et al. Effect of particle size on conformation and enzymatic activity of EcoRI adsorbed on CdS nanoparticles[J]. Colloids and Surfaces B-Biointerfaces,2014(114):269-276.
APA Song Y. H..,Zhong D. D..,Luo D..,Huang M..,Huang Z. Z..,...&Wang L..(2014).Effect of particle size on conformation and enzymatic activity of EcoRI adsorbed on CdS nanoparticles.Colloids and Surfaces B-Biointerfaces(114),269-276.
MLA Song Y. H.,et al."Effect of particle size on conformation and enzymatic activity of EcoRI adsorbed on CdS nanoparticles".Colloids and Surfaces B-Biointerfaces .114(2014):269-276.
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