Homogeneous Phase Fluorescence Assay Based on NaYF4 : Er3+, Yb3+ UCNPs as Donors

	Homogeneous Phase Fluorescence Assay Based on NaYF4 : Er3+, Yb3+ UCNPs as Donors
	Song K.; Zhang Q. B.; Zhao J. W.; Kong X. G.
	2013
发表期刊	Spectroscopy and Spectral Analysis
ISSN	ISBN/1000-0593
卷号	33 期号:4
摘要	Upconversion nanoparticles (UCNPs) of small size have great potential in homogeneous assay based on fluorescence resonance energy transfer (FRET). A novel approach of surface ligand-exchange for preparing water-soluble and amido-functionalized NaYF4 : Er3+, Yb3+ UCNPs with 12 nm was developed. The ligand exchange process was confirmed by Fourier transform infrared spectra. Investigations by scan electron microscopy showed no obvious variations in the size and shape of the UCNPs. The circular dichroism characterization demonstrates that the secondary structure of the avidin remains largely intact after the conjugation. Avidin-biotin served as a bridge to make the short enough distance for FRET between the acceptor biotinylated R-phycoerythrin and the donors avidin-conjugated UCNPs. When the free biotins were added into this system, they competitively combined with avidin on the UCNPs surface and impede the FRET to triggered fluorescence changes. According to the relationship between this change and the addition of the amount of biotin, such FRET-based approach can reach a limit of detection in the nanomolar concentration range.
收录类别	SCI
语种	中文
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/40695
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Song K.,Zhang Q. B.,Zhao J. W.,et al. Homogeneous Phase Fluorescence Assay Based on NaYF4 : Er3+, Yb3+ UCNPs as Donors[J]. Spectroscopy and Spectral Analysis,2013,33(4).
APA	Song K.,Zhang Q. B.,Zhao J. W.,&Kong X. G..(2013).Homogeneous Phase Fluorescence Assay Based on NaYF4 : Er3+, Yb3+ UCNPs as Donors.Spectroscopy and Spectral Analysis,33(4).
MLA	Song K.,et al."Homogeneous Phase Fluorescence Assay Based on NaYF4 : Er3+, Yb3+ UCNPs as Donors".Spectroscopy and Spectral Analysis 33.4(2013).

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