Enhancement of sensitivity and specificity of the fluoroimmunoassay of Hepatitis B virus surface antigen through "flexible" coupling between quantum dots and antibody

	Enhancement of sensitivity and specificity of the fluoroimmunoassay of Hepatitis B virus surface antigen through "flexible" coupling between quantum dots and antibody
其他题名	论文其他题名
	Zeng Q. H.; Zhang Y. L.; Song K.; Kong X. G.; Aalders M. C. G.; Zhang H.
	2009
发表期刊	Talanta
ISSN	0039-9140
卷号	80 期号:1 页码:307-312
摘要	Quantum dots (QDs) are widely used in the immune detection. Yet, the sensitivity and specificity of the immune detection are not satisfactory because the binding sites of QDs onto antibody (Ab) are often arbitrary and the influence of the large surface electronic potential energy of QDs on the directly conjugated Ab is nonnegligible. In this work, we provide a "flexible" coupling method, in which protein G (PG) is selected as the flexible bridge between the QDs and the Hepatitis B virus surface antibody (HBsAb), to improve the sensitivity and specificity of the fluoroimmunoassay compared to the directly covalent conjugation. Successful coupling of the HBsAb to our highly luminescent CdTe/CdS core/shell QDs is proven with Gel electrophoresis and atomic force microscopy (AFM). The assay results, based on the microelisa well plate as matrix to immobilize the sandwich structure, show that both sensitivity and specificity can be improved greatly through the flexible coupled QDs-PG-Ab conjugates. (C) 2009 Published by Elsevier B.V.
收录类别	SCI ; EI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/26286
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Zeng Q. H.,Zhang Y. L.,Song K.,et al. Enhancement of sensitivity and specificity of the fluoroimmunoassay of Hepatitis B virus surface antigen through "flexible" coupling between quantum dots and antibody[J]. Talanta,2009,80(1):307-312.
APA	Zeng Q. H.,Zhang Y. L.,Song K.,Kong X. G.,Aalders M. C. G.,&Zhang H..(2009).Enhancement of sensitivity and specificity of the fluoroimmunoassay of Hepatitis B virus surface antigen through "flexible" coupling between quantum dots and antibody.Talanta,80(1),307-312.
MLA	Zeng Q. H.,et al."Enhancement of sensitivity and specificity of the fluoroimmunoassay of Hepatitis B virus surface antigen through "flexible" coupling between quantum dots and antibody".Talanta 80.1(2009):307-312.

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