DNA adsorption and desorption on mica surface studied by atomic force microscopy

	DNA adsorption and desorption on mica surface studied by atomic force microscopy
其他题名	论文其他题名
	Sun L. L.; Zhao D. X.; Zhang Y.; Xu F. G.; Li Z. A.
	2011
发表期刊	Applied Surface Science
ISSN	0169-4332
卷号	257 期号:15 页码:6560-6567
摘要	The adsorption of DNA molecules on mica surface and the following desorption of DNA molecules at ethanol-mica interface were studied using atomic force microscopy. By changing DNA concentration, different morphologies on mica surface have been observed. A very uniform and orderly monolayer of DNA molecules was constructed on the mica surface with a DNA concentration of 30 ng/mu L. When the samples were immersed into ethanol for about 15 min, various desorption degree of DNA from mica (0-99%) was achieved. It was found that with the increase of DNA concentration, the desorption degree of DNA from the mica at ethanol-mica interface decreased. And when the uniform and orderly DNA monolayers were formed on the mica surface, almost no DNA molecule desorbed from the mica surface in this process. The results indicated that the uniform and orderly DNA monolayer is one of the most stable DNA structures formed on the mica surface. In addition, we have studied the structure change of DNA molecules after desorbed from the mica surface with atomic force microscopy, and found that the desorption might be ascribed to the ethanol-induced DNA condensation. (C) 2011 Elsevier B. V. All rights reserved.
收录类别	SCI ; EI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/26058
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Sun L. L.,Zhao D. X.,Zhang Y.,et al. DNA adsorption and desorption on mica surface studied by atomic force microscopy[J]. Applied Surface Science,2011,257(15):6560-6567.
APA	Sun L. L.,Zhao D. X.,Zhang Y.,Xu F. G.,&Li Z. A..(2011).DNA adsorption and desorption on mica surface studied by atomic force microscopy.Applied Surface Science,257(15),6560-6567.
MLA	Sun L. L.,et al."DNA adsorption and desorption on mica surface studied by atomic force microscopy".Applied Surface Science 257.15(2011):6560-6567.

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