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Tb3+ as fluorescent probe in the study of the interaction of other lanthanide ions with trichosanthin
其他题名论文其他题名
Hao Q.; Li W. L.; Zhang Y.; Yu G.; Yang H.; Zhao X.; Yao Q. Z.; Li Q. S.
1995
发表期刊Chemical Research in Chinese Universities
ISSN1005-9040
卷号11期号:4页码:279-283
摘要The interaction of lanthanide(Ln(3+)) ions with trichosanthin(TCS) was investigated using Tb3+ as fluorescent probe. The metal-binding sites of the protein were probed by means of adding the Tb3+ into the protein solutions. If other Ln(3+) ions exist in the Tb3+-protein complex system, they would compete against Tb3+ in the binding sites of the protein, and so the fluorescence intensity of Tb3+ decreased, which is called quenching effect. The quenching effect is related to the ionic radii of the Ln(3+) ions and energy transfer from Tb3+-protein to Ln(3+) ions. Based on the experimental results, a schematic of the energy transfer from protein-Tb3+ complex to protein-Ln(3+) complex is suggested.
收录类别SCI
语种英语
文献类型期刊论文
条目标识符http://ir.ciomp.ac.cn/handle/181722/25974
专题中科院长春光机所知识产出
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GB/T 7714
Hao Q.,Li W. L.,Zhang Y.,et al. Tb3+ as fluorescent probe in the study of the interaction of other lanthanide ions with trichosanthin[J]. Chemical Research in Chinese Universities,1995,11(4):279-283.
APA Hao Q..,Li W. L..,Zhang Y..,Yu G..,Yang H..,...&Li Q. S..(1995).Tb3+ as fluorescent probe in the study of the interaction of other lanthanide ions with trichosanthin.Chemical Research in Chinese Universities,11(4),279-283.
MLA Hao Q.,et al."Tb3+ as fluorescent probe in the study of the interaction of other lanthanide ions with trichosanthin".Chemical Research in Chinese Universities 11.4(1995):279-283.
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