Changchun Institute of Optics,Fine Mechanics and Physics,CAS
Preparation, Characterization and Specific Biological Labeling of Silica Coated Upconversion Fluorescent Nanocrystals | |
其他题名 | 论文其他题名 |
Song K.; Tian L. J.; Kong X. G.; Liu K.; Zhang Q. B.; Du C.; Zeng Q. H.; Sun Y. J.; Liu X. M. | |
2010 | |
发表期刊 | Spectroscopy and Spectral Analysis |
ISSN | 1000-0593 |
卷号 | 30期号:1页码:133-136 |
摘要 | The authors synthesized a kind of upconversion nanocrystals NaYF(4) : Yb(3+), Er(3+) via the hydrothermal assisted homogeneous precipitation method, and then the nanocrystal was coated with silica. The SEM image demonstrated that the as-prepared samples were uniform in size distribution with ca. 25 nm before and ca. 250 nm after silica coating, respectively. The upconversion spectra and photoluminescence lifetime measurement showed that the silica shell had hardly effect on the properties of fluorescence of the NaYF(4) : Yb(3+), Er(3+) nanocrystals. At the same time, the naked eye-visible green upconversion fluorescence pattern was acquired from the as-prepared upconversion nanoparticles in the PBS buffer (2 wt%) excited by 980 nm laser at room temperature. These water-soluble nanoparticles were linked to the antibodies using the coupling reagents glutaraldehyde. The circular dichroism (CD) spectra of antibody and upconversion nanoparticles-antibody conjugates were very similar to each other, indicating that the secondary structure of antibody remained largely intact after the conjugation Finally, antigen-antibody recognition reaction was performed on the surface of a silicon slide. The immunofluorescence in vitro indicated that the upconversion nanoparticles-antibody bioconjugates had excellent species-specific detection ability with hardly non-specific binding. Based on the present results, it is anticipated that the silica-coated upconversion nanoparticles are suitable for use as biolabeling materials. |
收录类别 | SCI |
语种 | 中文 |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ciomp.ac.cn/handle/181722/25609 |
专题 | 中科院长春光机所知识产出 |
推荐引用方式 GB/T 7714 | Song K.,Tian L. J.,Kong X. G.,et al. Preparation, Characterization and Specific Biological Labeling of Silica Coated Upconversion Fluorescent Nanocrystals[J]. Spectroscopy and Spectral Analysis,2010,30(1):133-136. |
APA | Song K..,Tian L. J..,Kong X. G..,Liu K..,Zhang Q. B..,...&Liu X. M..(2010).Preparation, Characterization and Specific Biological Labeling of Silica Coated Upconversion Fluorescent Nanocrystals.Spectroscopy and Spectral Analysis,30(1),133-136. |
MLA | Song K.,et al."Preparation, Characterization and Specific Biological Labeling of Silica Coated Upconversion Fluorescent Nanocrystals".Spectroscopy and Spectral Analysis 30.1(2010):133-136. |
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