Simultaneous determination of three bioactive homoisoflavanones in rhizomes of Polygonatum odoratum

	Simultaneous determination of three bioactive homoisoflavanones in rhizomes of Polygonatum odoratum
其他题名	论文其他题名
	Wang W.; Shi H. B.; Zhu R. N.; Zhang D. W.; Han Y.; Sun T. T.
	2011
发表期刊	Journal of Medicinal Plants Research
ISSN	1996-0875
卷号	5 期号:20 页码:5184-5190
摘要	An high-performance liquid chromatographic- photodiode array detector method (HPLC-PAD) method was established to simultaneously determine three bioactive homoisoflavanones in the rhizomes of Polygonatum odoratum (Mill) Druce. The optimal conditions of extraction were achieved using reflux extraction with methanol-water (80: 20, v/v) for 2 h. The analysis was performed on a Diamonsil ODS column with a gradient elution of methanol and 1% acetic acid aqueous solution at a flow rate of 1.0 ml/min. The detection wavelength was 297 nm and the column temperature was 30 degrees C. Linear relationships within the range of investigated concentrations were observed for three bioactive homoisoflavanones with their correlation coefficients greater than 0.9999. The method was validated for intra-and inter-day precision (RSD < 2.05%), stability (RSD < 2.40%), and repeatability (RSD < 2.38%) with recovery between 98.67 and 100.52% and the RSD less than 2.28%. The method was successfully applied to simultaneously determine three homoisoflavanones in the twelve samples of Rhizoma Polygonati Odorati collected from different regions. The results indicated that the developed assay could be considered as a suitable quality control method for the rhizomes of P. odoratum.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/25597
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Wang W.,Shi H. B.,Zhu R. N.,et al. Simultaneous determination of three bioactive homoisoflavanones in rhizomes of Polygonatum odoratum[J]. Journal of Medicinal Plants Research,2011,5(20):5184-5190.
APA	Wang W.,Shi H. B.,Zhu R. N.,Zhang D. W.,Han Y.,&Sun T. T..(2011).Simultaneous determination of three bioactive homoisoflavanones in rhizomes of Polygonatum odoratum.Journal of Medicinal Plants Research,5(20),5184-5190.
MLA	Wang W.,et al."Simultaneous determination of three bioactive homoisoflavanones in rhizomes of Polygonatum odoratum".Journal of Medicinal Plants Research 5.20(2011):5184-5190.

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