A New Optical Switch Using Upconversion Nanoparticles Conjugated Aptamer

	A New Optical Switch Using Upconversion Nanoparticles Conjugated Aptamer
其他题名	论文其他题名
	Song K.; Kong X. G.
	2011
发表期刊	Spectroscopy and Spectral Analysis
ISSN	1000-0593
卷号	31 期号:3 页码:844-848
摘要	As new class of fluorescent biolabels, upconversion nanoparticles (UCNPs) were very attractive for use as labels, compared with these traditional downconversion materials. It can eliminate problems associated with autofluorescence and scattered excitation light under near infrared light (NIR) excitation. In the present work, water-soluble NaYF(4) : Yb(3+), Er(3+) UC-NPs were successfully prepared by solvothermal synthesis, using branched polyethylenimine as the surfactants, and a generic design strategy for UCNPs conjugated aptamer based optical switch was presented. In the absence of the target thrombin protein, such a duplex structure brings the UCNPs and TAMRA close proximity of each other, leading to luminescence resonance energy transfer from UCNPs to TAMRA upon near-infrared light irradiation. When target is introduced, the quadruplex conformation of the aptamer should be preferentially stabilized, resulting in the displacement of the TAMRA labelled oligonucleotide conjugate and triggering UCNPs fluorescence intensity to increase and the corresponding TAMRA fluorescence intensity to decrease. Since the aptamer responds to its target molecule by switching on its fluorescent properties, the authors named this an "optical switch".
收录类别	SCI
语种	中文
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/25589
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Song K.,Kong X. G.. A New Optical Switch Using Upconversion Nanoparticles Conjugated Aptamer[J]. Spectroscopy and Spectral Analysis,2011,31(3):844-848.
APA	Song K.,&Kong X. G..(2011).A New Optical Switch Using Upconversion Nanoparticles Conjugated Aptamer.Spectroscopy and Spectral Analysis,31(3),844-848.
MLA	Song K.,et al."A New Optical Switch Using Upconversion Nanoparticles Conjugated Aptamer".Spectroscopy and Spectral Analysis 31.3(2011):844-848.

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