Purification and properties of carboxymethylcellulase (CMCase) from a cellulose-decomposing bacterium Sporocytophaga sp JL-01

	Purification and properties of carboxymethylcellulase (CMCase) from a cellulose-decomposing bacterium Sporocytophaga sp JL-01
其他题名	论文其他题名
	Wang X. R.; Liu D. B.; Peng Z. Q.; Chen S.; Lu T. C.; Li F.; Xia H. M.
	2012
发表期刊	African Journal of Microbiology Research
ISSN	1996-0808
卷号	6 期号:14 页码:3528-3533
摘要	Sporocytophaga sp., a Gram-negative aerobic cellulose decomposing bacterium, is capable of gliding on the surface of solid medium. A new endoglucanase, carboxymethylcellulase (CMCase1), was purified from an aerobic bacterium Sporocytophaga sp. JL-01. The molecular weight of the enzyme was 82 kDa as determined by SDS Polyacrylamide gel electrophoresis (SDS-PAGE). The optimum pH was 5.0 and optimum temperature was 50 degrees C under experiment conditions. The Km of CMCase1 was 9 mgmL(-1) and Vmax was 27.3 ug min(-1)mg(-1) protein. The Zn2+ could inhibit the activity of this enzyme at ion concentration of 5 mM. RP-HPLC revealed that aromatic amino acid, acidic amino acid, and basic amino acid accounted for 10.5, 24.5 and 7.0%, respectively. The far-UV circular dichroism (CD) spectrum showed that the amount of beta-sheet was decreased with the temperature changed from 25 to 75 degrees C.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/25552
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Wang X. R.,Liu D. B.,Peng Z. Q.,et al. Purification and properties of carboxymethylcellulase (CMCase) from a cellulose-decomposing bacterium Sporocytophaga sp JL-01[J]. African Journal of Microbiology Research,2012,6(14):3528-3533.
APA	Wang X. R..,Liu D. B..,Peng Z. Q..,Chen S..,Lu T. C..,...&Xia H. M..(2012).Purification and properties of carboxymethylcellulase (CMCase) from a cellulose-decomposing bacterium Sporocytophaga sp JL-01.African Journal of Microbiology Research,6(14),3528-3533.
MLA	Wang X. R.,et al."Purification and properties of carboxymethylcellulase (CMCase) from a cellulose-decomposing bacterium Sporocytophaga sp JL-01".African Journal of Microbiology Research 6.14(2012):3528-3533.

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