Monitoring HSV-TK/ganciclovir cancer suicide gene therapy using CdTe/CdS core/shell quantum dots

	Monitoring HSV-TK/ganciclovir cancer suicide gene therapy using CdTe/CdS core/shell quantum dots
其他题名	论文其他题名
	Shao D.; Zeng Q. H.; Fan Z.; Li J.; Zhang M.; Zhang Y. L.; Li O.; Chen L.; Kong X. G.; Zhang H.
	2012
发表期刊	Biomaterials
ISSN	0142-9612
卷号	33 期号:17 页码:4336-4344
摘要	To be able to label a gene and monitor its migration are key important approaches for the clinical application of cancer suicide gene therapy. Photonic nanomaterials are introduced in this work. One of the most promised suicide genes - herpes simplex virus thymidine kinase (HSV-TK) gene - is successfully linked with CdTe/CdS core/shell quantum dots (QDs) via EDC/NHS coupling method. From confocal microscopy it was demonstrated that plasmid TK intracellular trafficking can be effectively and distinctly traced via monitoring the luminescence of the QDs up to 96 h after transfection of QDs-TK conjugates into Hela cells. MTT results show that the QDs-TK conjugates have a high efficient cytotoxicity after adding GCV into Hela cells, whereas the QDs exert no detectable deleterious effects on the cellular processes. The apoptosis induced by QDs-TK conjugates with GCV is distinctly traced partly due to the strong luminescence of the QDs. Our results indicate that photonic nanomaterials, e.g. QDs, provide a tool for monitoring TK gene delivery and anti-cancer activity. (C) 2012 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/24611
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Shao D.,Zeng Q. H.,Fan Z.,et al. Monitoring HSV-TK/ganciclovir cancer suicide gene therapy using CdTe/CdS core/shell quantum dots[J]. Biomaterials,2012,33(17):4336-4344.
APA	Shao D..,Zeng Q. H..,Fan Z..,Li J..,Zhang M..,...&Zhang H..(2012).Monitoring HSV-TK/ganciclovir cancer suicide gene therapy using CdTe/CdS core/shell quantum dots.Biomaterials,33(17),4336-4344.
MLA	Shao D.,et al."Monitoring HSV-TK/ganciclovir cancer suicide gene therapy using CdTe/CdS core/shell quantum dots".Biomaterials 33.17(2012):4336-4344.

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